PCR
In PCR, the temperatures are 90-95°C for denaturation, 42-55°C for annealing, and 70-75°C for extension right? So answer is supposed to be A? Why is it given to be B?
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@Sheldon J. Plankton
Note for OP
+solved @user1 @user2... to close the thread when your doubt is solved. Mention the users who helped you solve the doubt. This will be added to their stats.which book?
?
which book is this from?
Oh, this is a NEST 2024 pyq
so, uh, when you swap out taq polymerase for the e. coli version in your pcr, you kinda have to, you know, treat it like a person with its own comfort zone. taq is the hardcore type that is cool with 72 degree c, basically a sauna, whereas e. coli polymerase is more like your average human who faints if you crank the heat too high. if you tried to run extension at taq is 72 degree c, the e. coli enzyme would just... fall apart. and if you dial it down to 25 degree c, it is like asking someone to run a marathon in flip flops, it is way too slow. so you settle on around 37 degree c, which is basically the enzyme’s home sweet home temperature, where it is happy, folded up nicely, and ready to crank out dna before the next denaturation step.
Ok, but how do we know that E.coli is better at 37 than 25?
I can’t tell if you wrote this or a bot did. If it was you, hats off XD
😭
Human body temp.
Oh
e. coli lives at body temperature its polymerase has evolved to be most active around that range so the chemical reactions happen faster there.
yeah
+ isnt it a mesophilic enzyme?
It's in Biotech chapter tho
😭
+solved @TK @iTeachChem
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