10 Replies
@Dexter
Note for OP
+solved @user1 @user2... to close the thread when your doubt is solved. Mention the users who helped you solve the doubt. This will be added to their stats.electrophoreiosis me ik machine me electric field generate karte hai aur 2 plates hoti hai ik negative aur ik positive
usually negative ke side par sample load karte hai aur dekhte hai ki charge ke basis pe kon move karenga ya nhi aur size aur charge pe dekhte hai ki kitna move karega
us point of view se socho
kyunki mujhe ispe itna hi aata hai abhi
iteachchem
Transcription requested by Sephrina
Hey, you gotta find the isoelectronic point.
yeah that ik but idk what pka values to use
i dont see how this can be applied here
at 3 it is protonated
at 11 it is -ve
Not even at 7 its gonna be protonated
so you use (7 + 11)/2
<7 ie
cos 7 and 11 are amine ke pka values
pkb is 7 and 3 there
hol up, removing a few things on top :)
basically somewhere in between where teh charge changes sign
https://www.masterorganicchemistry.com/2023/02/09/isoelectric-point-calculation/
see this actually. Point 5
James Ashenhurst
Master Organic Chemistry
Isoelectric Points of Amino Acids (and How To Calculate Them)
The isoelectric point of an amino acid is the pH at which it bears a net charge of zero. It can be calculated through averaging the two pka values
Ans is 9
Will give this a read once i get back home
yea 11 + 7 by 2
the two pkas of the basic chains