pBR322
I know not like many people are there but does anyone like understand what happened in the second step?
4 Replies
@Sheldon J. Plankton
Note for OP
+solved @user1 @user2... to close the thread when your doubt is solved. Mention the users who helped you solve the doubt. This will be added to their stats.Are we taking cells from the ampicillin resistant colonies and transferring to tetracycline medium to check if they are recombinant or not?
yes
eggjacktly yehi krre h
the process shown in the diagram is known as replica plating, and it is used to identify recombinant colonies in a bacterial transformation experiment using antibiotic resistance markers